Emergence of highly virulent multidrug and extensively drug resistant Escherichia coli and Klebsiella pneumoniae in buffalo subclinical mastitis cases

The study analyzed virulence and antibiotic resistance genes in Escherichia coli and Klebsiella pneumoniae from subclinical mastitis cases in buffaloes. The prevalence of SCM was 67.9% at the quarter level and 80.8% at the animal level. Virulence genes, resistance genes, and β-lactamase genes were detected. Biofilm formation was found in 83.6% of E. coli isolates and 75.7% of K. pneumoniae isolates. MDR was observed in 31.5% of E. coli and 39.3% of K. pneumoniae isolates.